During the academic year April to December 2021, I have collected strong imaging and electrophysiology data to support the manuscript “Activation of leptin receptor positive neurons in dorsomedial hypothalamus stimulates breathing in obese mice”. I have made good progress in trouble shooting: adjusting dissection orientation to obtain brain slices that contain sufficient neural pathway connections between two separate and long-distance neuron populations for electrophysiology study, patch clamp neurons successfully in old animal, and IHC staining to identify the specific 5HT cells. Our finding confirms that central Leptin receptor-b (LepR+) containing neurons project extensively to the serotonin neurons in different areas including midbrain and medulla. Optogenetic activation of LepR+ ChR2 positive fibers in the dorsal raphe 5HT cells of LepR-Cre-ChR2 mice evoked a corresponding excitatory postsynaptic current in serotonin neurons. This may be a mechanism that leptin reduces obstructive sleep apnea and maintain respiratory function in obese patients.