My principle research focus over the past 30 years has been related to the regulation of lymphocyte activation and differentiation. As Medical Director of the Clinical Flow Cytometry and Immunology Diagnostic laboratory at Children's National Health System, and during a sabbatical experience at the Center of Human Immunology at the NIH, I also have gained significant expertise in the development and application of assays to assess human immune status. Recently, I have contributed to the development of several translational and clinical research proposals that require expertise in assessment of biomarkers related to assessment of human immune status. Further, these experiences have facilitated the development and enhancement of leadership roles in graduate student and medical student education. Over the past several years I have become more committed to education of medical students, and have served as Interim Assistant Dean of Pre-clinical education, and continue to serve as Chair of the Pre-clinical curriculum committee. This year, I continued in my role as Director of Medical Student Research and Co-director of the Clinical and Translational Research scholarly concentration. I also took on the additional role as co-leader of MD-Clinical Research Practice certificate program. I am also course co-director for two courses in the pre-clinical phase of the medical student curriculum, and personally teach Immunology related content throughout the pre-clinical curriculum as well as in support of Pathology resident graduate medical education. I am also active in PhD program educational activities. I am co-director of the PhD program in Microbiology and Immunlogy and serve as Chair of the Committee on Graduate Research and Post-doctoral Programs. I am also course director for two immunology courses designed for masters and PhD level students. In addition, I serve on multiple student PhD thesis committees and provide mentoring and advice to undergraduate medical students as well as pathology residents. As noted above, I serve as Medical Director of the Immunology and Flow Cytometry laboratory at Children's National. This is primarily a service role, where I oversee flow cytometry-based clinical diagnostic testing related to immunodeficiency and hematologic disorders. This includes the provision of consultative and professional interpretation service in evaluating patients with suspected immunodeficiency disorders, as well as oversight of flow cytometric diagnostic testing in support of the hematopoietic stem cell transplant service.